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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NAB2 Polyclonal antibody

NAB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 19601-1-AP

Synonyms

Protein MADER, NGFI-A-binding protein 2, Nab 2, Melanoma-associated delayed early response protein, MADER

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using NAB2 Polyclonal antibody (19601-1-AP)

    WB analysis of human heart using 19601-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 19601-1-AP (NAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using NAB2 Polyclonal antibody (19601-1-AP)

    WB analysis of K-562 using 19601-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 19601-1-AP (NAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using NAB2 Polyclonal antibody (19601-1-AP)

    WB analysis of mouse thymus using 19601-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19601-1-AP (NAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NAB2 Polyclonal antibody (19601-1-AP)

    WB analysis of human brain using 19601-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19601-1-AP (NAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using NAB2 Polyclonal antibody (19601-1-AP)

    IP experiment of K-562 using 19601-1-AP

    IP result of anti-NAB2 (IP:19601-1-AP, 4ug; Detection:19601-1-AP 1:700) with K-562 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using NAB2 Polyclonal antibody (19601-1-AP)

    IHC staining of human brain using 19601-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19601-1-AP (NAB2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using NAB2 Polyclonal antibody (19601-1-AP)

    IHC staining of human brain using 19601-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19601-1-AP (NAB2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NAB2 Polyclonal antibody (19601-1-AP)

    IHC staining of human kidney using 19601-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19601-1-AP (NAB2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NAB2 Polyclonal antibody (19601-1-AP)

    IHC staining of human kidney using 19601-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19601-1-AP (NAB2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NAB2 Polyclonal antibody (19601-1-AP)

    IF Staining of MCF-7 using 19601-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 19601-1-AP (NAB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman heart tissue, human brain tissue, K-562 cells, mouse thymus tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19601-1-AP targets NAB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAB2 fusion protein Ag6346 相同性解析による交差性が予測される生物種
Full Name NGFI-A binding protein 2 (EGR1 binding protein 2)
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC065931
Gene symbol NAB2
Gene ID (NCBI) 4665
RRIDAB_10640524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NAB2 antibody 19601-1-APDownload protocol
IHC protocol for NAB2 antibody 19601-1-APDownload protocol
IF protocol for NAB2 antibody 19601-1-APDownload protocol
IP protocol for NAB2 antibody 19601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Invest Ophthalmol Vis Sci

β1-Integrin Deletion From the Lens Activates Cellular Stress Responses Leading to Apoptosis and Fibrosis.

Authors - Yichen Wang
View Article
mouseWB

Lab Anim (NY)

Early growth response 2 in the mPFC regulates mouse social and cooperative behaviors

Authors - Yanli Zhang
View Article