Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of human heart using 19601-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 19601-1-AP (NAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 19601-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 19601-1-AP (NAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 19601-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19601-1-AP (NAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 19601-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 19601-1-AP (NAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 19601-1-AP
IP result of anti-NAB2 (IP:19601-1-AP, 4ug; Detection:19601-1-AP 1:700) with K-562 cells lysate 3200ug.
IHC staining of human brain using 19601-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 19601-1-AP (NAB2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 19601-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 19601-1-AP (NAB2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 19601-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 19601-1-AP (NAB2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 19601-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 19601-1-AP (NAB2 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of MCF-7 using 19601-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NAB2 antibody (19601-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red).

NAB2 Polyclonal antibody

NAB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 19601-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Protein MADER, NGFI-A-binding protein 2, Nab 2, Melanoma-associated delayed early response protein, MADER

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human heart tissue, human brain tissue, K-562 cells, mouse thymus tissue
Positive IP detected in	K-562 cells
Positive IHC detected in	human brain tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IF	See 1 publications below

Product Information

19601-1-AP targets NAB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NAB2 fusion protein Ag6346 相同性解析による交差性が予測される生物種
Full Name	NGFI-A binding protein 2 (EGR1 binding protein 2)
Calculated molecular weight	57 kDa
Observed molecular weight	57 kDa
GenBank accession number	BC065931
Gene Symbol	NAB2
Gene ID (NCBI)	4665
RRID	AB_10640524
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15742
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for NAB2 antibody 19601-1-AP	Download protocol
IHC protocol for NAB2 antibody 19601-1-AP	Download protocol
IF protocol for NAB2 antibody 19601-1-AP	Download protocol
IP protocol for NAB2 antibody 19601-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Invest Ophthalmol Vis Sci β1-Integrin Deletion From the Lens Activates Cellular Stress Responses Leading to Apoptosis and Fibrosis. Authors - Yichen Wang View Article
mouse	WB	Lab Anim (NY) Early growth response 2 in the mPFC regulates mouse social and cooperative behaviors Authors - Yanli Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human heart using 19601-1-AP

WB analysis of K-562 using 19601-1-AP

WB analysis of mouse thymus using 19601-1-AP

WB analysis of human brain using 19601-1-AP

IP experiment of K-562 using 19601-1-AP

IHC staining of human brain using 19601-1-AP