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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NADK Polyclonal antibody (29290-1-AP)

    WB analysis using 29290-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29290-1-AP (NADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NADK Polyclonal antibody (29290-1-AP)

    IHC staining of human stomach cancer using 29290-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29290-1-AP (NADK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using NADK Polyclonal antibody (29290-1-AP)

    IHC staining of human ovary cancer using 29290-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29290-1-AP (NADK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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NADK Polyclonal antibody

NADK Polyclonal Antibody for WB, ELISA
Cat No. 29290-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

dJ283E3.1, FLJ13052, FLJ37724, NAD kinase, NADK, Poly(P)/ATP NAD kinase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, Hela cells, Neuro-2a cells, NIH/3T3 cells, rat kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29290-1-AP targets NADK in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NADK fusion protein Ag30982 相同性解析による交差性が予測される生物種
Full Name NAD kinase
Calculated molecular weight 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC001709
Gene Symbol NADK
Gene ID (NCBI) 65220
RRIDAB_2918272
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95544
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).

Protocols

Product Specific Protocols
WB protocol for NADK antibody 29290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols