• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

APPBP1 Polyclonal antibody

APPBP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14863-1-AP

Synonyms

NAE1, HPP1, HPP 1, APP-BP1, APP BP1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human kidney tissue using APPBP1 Polyclonal antibody (14863-1-AP)

    WB analysis of human kidney using 14863-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 14863-1-AP (APPBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using APPBP1 Polyclonal antibody (14863-1-AP)

    WB analysis of mouse brain using 14863-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14863-1-AP (APPBP1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using APPBP1 Polyclonal antibody (14863-1-AP)

    WB analysis of human brain using 14863-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14863-1-AP (APPBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using APPBP1 Polyclonal antibody (14863-1-AP)

    IP experiment of mouse kidney using 14863-1-AP

    IP result of anti-APPBP1 (IP:14863-1-AP, 4ug; Detection:14863-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of mouse salivary gland tissue using APPBP1 Polyclonal antibody (14863-1-AP)

    IHC staining of mouse salivary gland using 14863-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using 14863-1-AP (APPBP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inhuman kidney tissue, human brain tissue, mouse brain tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inmouse salivary gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14863-1-AP targets APPBP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APPBP1 fusion protein Ag6672 相同性解析による交差性が予測される生物種
Full Name NEDD8 activating enzyme E1 subunit 1
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC000480
Gene symbol NAE1/APPBP1
Gene ID (NCBI) 8883
RRIDAB_2251388
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for APPBP1 antibody 14863-1-APDownload protocol
IHC protocol for APPBP1 antibody 14863-1-APDownload protocol
IP protocol for APPBP1 antibody 14863-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Proc Natl Acad Sci U S A

Polo-like kinases mediate cell survival in mitochondrial dysfunction.

Authors - Takumi Matsumoto
  • KD Validated
View Article