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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NAGS Recombinant antibody, PBS Only

NAGS Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230279D3

Cat no : 82963-1-PBS

Synonyms

N-acetylglutamate synthase conserved domain form, EC:2.3.1.1, ARGA, Amino-acid acetyltransferase, AGAS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAGS Recombinant antibody (82963-1-RR)

    WB analysis using 82963-1-RR (same clone as 82963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82963-1-RR (NAGS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82963-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82963-1-RR

    Affinity and Kinetic Characterization of 82963-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82963-1-RR against Human NAGS were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82963-1-PBS targets NAGS in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NAGS fusion protein Ag16073 相同性解析による交差性が予測される生物種
Full Name N-acetylglutamate synthase
Calculated molecular weight 534 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC111713
Gene symbol NAGS
Gene ID (NCBI) 162417
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

N-acetylglutamate synthase, also known as NAGS, is a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAGS is the key enzyme for regulating the hepatic urea cycle and is also highly expressed in the kidney and gut. Deficiency of NAGS leads to hyperammonemia due to decreased activity of CPSI deprived of its cofactor NAG(PMID:12459178,12754705).