Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 11776-1-AP
Rat liver lysates were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of Ramos using 11776-1-AP
Ramos cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of RAW 264.7 using 11776-1-AP
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Ramos using 11776-1-AP
Ramos cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11776-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 11776-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 11776-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 11776-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of zebrafish using 11776-1-AP
zebrafish tissue were subjected to SDS PAGE followed by western blot with 11776-1-AP (NAMPT/PBEF antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 11776-1-AP
IP result of anti-NAMPT/PBEF (IP:11776-1-AP, 4ug; Detection:11776-1-AP 1:1000) with mouse heart tissue lysate 3720 ug.
IP experiment of mouse heart using 11776-1-AP
IP result of anti-NAMPT/PBEF (IP:11776-1-AP, 4ug; Detection:11776-1-AP 1:4000) with mouse heart tissue lysate 1560 ug.
IHC staining of human lymphoma using 11776-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 11776-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 11776-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NAMPT/PBEF antibody (11776-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of U-87 MG using 11776-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using NAMPT/PBEF antibody (11776-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 11776-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NAMPT/PBEF antibody (11776-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

NAMPT/PBEF Polyclonal antibody

NAMPT/PBEF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11776-1-AP

Publications(49)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Nampt, Visfatin, Pre B cell enhancing factor, PBEF1, PBEF

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (49)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	rat liver tissue, Ramos cells, mouse heart tissue, HL-60 cells, RAW 264.7 cells, mouse liver tissue, mouse skeletal muscle tissue
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells, HeLa cells, U-87 MG cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 45 publications below
IHC	See 11 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

11776-1-AP targets NAMPT/PBEF in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity	human, mouse, rat, zebrafish
Cited Reactivity	human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NAMPT/PBEF fusion protein Ag2434 相同性解析による交差性が予測される生物種
Full Name	nicotinamide phosphoribosyltransferase
Calculated molecular weight	52 kDa
Observed molecular weight	50-55 kDa
GenBank accession number	BC020691
Gene Symbol	NAMPT/PBEF
Gene ID (NCBI)	10135
RRID	AB_2298317
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P43490
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.

Protocols

Product Specific Protocols
WB protocol for NAMPT/PBEF antibody 11776-1-AP	Download protocol
IHC protocol for NAMPT/PBEF antibody 11776-1-AP	Download protocol
IF protocol for NAMPT/PBEF antibody 11776-1-AP	Download protocol
IP protocol for NAMPT/PBEF antibody 11776-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Cell Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage. Authors - Krishna S Tummala View Article
human,mouse	IHC,WB	Cell Metab NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion. Authors - Hongwei Lv KD Validated View Article
mouse	WB,IHC	Cell Death Dis Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy Authors - Yang Deng View Article
human,mouse	WB,IHC	Int J Mol Sci NAD+ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy. Authors - Xue Li View Article
human,mouse	IF,WB	Metabolism Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice. Authors - Ya Li KD Validated View Article
human	WB	Oncogenesis Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) with OT-82 induces DNA damage, cell death, and suppression of tumor growth in preclinical models of Ewing sarcoma. Authors - Anna E Gibson KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11776-1-AP

WB analysis of Ramos using 11776-1-AP

WB analysis of RAW 264.7 using 11776-1-AP

WB analysis of Ramos using 11776-1-AP

WB analysis of mouse liver using 11776-1-AP

WB analysis of mouse heart using 11776-1-AP

WB analysis of HL-60 using 11776-1-AP

WB analysis of mouse skeletal muscle using 11776-1-AP

WB analysis of zebrafish using 11776-1-AP

IP experiment of mouse heart using 11776-1-AP

IP experiment of mouse heart using 11776-1-AP

IHC staining of human lymphoma using 11776-1-AP

IHC staining of human lymphoma using 11776-1-AP

IF Staining of HEK-293 using 11776-1-AP

IF Staining of U-87 MG using 11776-1-AP

IF Staining of HeLa using 11776-1-AP

NAMPT/PBEF Polyclonal antibody

NAMPT/PBEF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.

NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion.

Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy

NAD+ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy.

Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice.

Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) with OT-82 induces DNA damage, cell death, and suppression of tumor growth in preclinical models of Ewing sarcoma.