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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAMPT/PBEF Monoclonal antibody (66385-1-Ig)

    WB analysis using 66385-1-Ig (same clone as 66385-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NAMPT/PBEF Monoclonal antibody (66385-1-Ig)

    WB analysis using 66385-1-Ig (same clone as 66385-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using NAMPT/PBEF Monoclonal antibody (66385-1-Ig)

    WB analysis of Neuro-2a using 66385-1-Ig (same clone as 66385-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using NAMPT/PBEF Monoclonal antibody (66385-1-Ig)

    WB analysis of human heart using 66385-1-Ig (same clone as 66385-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using NAMPT/PBEF Monoclonal antibody (66385-1-Ig)

    WB analysis of human skeletal muscle using 66385-1-Ig (same clone as 66385-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using NAMPT/PBEF Monoclonal antibody (66385-1-Ig)

    WB analysis of rat heart using 66385-1-Ig (same clone as 66385-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using NAMPT/PBEF Monoclonal antibody (66385-1-Ig)

    WB analysis of mouse skeletal muscle using 66385-1-Ig (same clone as 66385-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NAMPT/PBEF Monoclonal antibody (66385-1-Ig)

    IHC staining of human breast cancer using 66385-1-Ig (same clone as 66385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NAMPT/PBEF Monoclonal antibody (66385-1-Ig)

    IHC staining of human breast cancer using 66385-1-Ig (same clone as 66385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NAMPT/PBEF Monoclonal antibody (66385-1-Ig)

    IF Staining of HeLa using 66385-1-Ig (same clone as 66385-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAMPT/PBEF antibody (66385-1-Ig, Clone: 3D4D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NAMPT/PBEF Monoclonal antibody (66385-1-Ig)

    IF Staining of HepG2 using 66385-1-Ig (same clone as 66385-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NAMPT/PBEF antibody (66385-1-Ig, Clone: 3D4D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

NAMPT/PBEF Monoclonal antibody, PBS Only

NAMPT/PBEF Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66385-1-PBS
Clone No.3D4D8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1110035O14Rik, NAmPRTase, NAMPT, NAMPT/PBEF, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66385-1-PBS targets NAMPT/PBEF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NAMPT/PBEF fusion protein Ag2434 相同性解析による交差性が予測される生物種
Full Name nicotinamide phosphoribosyltransferase
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC020691
Gene Symbol NAMPT/PBEF
Gene ID (NCBI) 10135
RRIDAB_2881761
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43490
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.