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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NANP Polyclonal antibody

NANP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12519-1-AP

Synonyms

Neu5Ac-9-Pase, N-acylneuraminate-9-phosphatase, HDHD4, Haloacid dehalogenase-like hydrolase domain-containing protein 4, EC:3.1.3.29

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NANP Polyclonal antibody (12519-1-AP)

    WB analysis using 12519-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12519-1-AP (NANP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using NANP Polyclonal antibody (12519-1-AP)

    IHC staining of human ovary cancer using 12519-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12519-1-AP (NANP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NANP Polyclonal antibody (12519-1-AP)

    IF Staining of A431 using 12519-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using NANP antibody (12519-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inK-562 cells, Neuro-2a cells, mouse brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12519-1-AP targets NANP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NANP fusion protein Ag3212 相同性解析による交差性が予測される生物種
Full Name N-acetylneuraminic acid phosphatase
Calculated molecular weight 248 aa, 28 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC022552
Gene symbol NANP
Gene ID (NCBI) 140838
RRIDAB_3085396
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NANP, also known as HDHD4, C20orf147, is a member of the haloacid dehalogenase family of proteins. NANP enables N-acylneuraminate-9-phosphatase activity and is involved in N-acetylneuraminate biosynthetic process.

Protocols

Product Specific Protocols
WB protocol for NANP antibody 12519-1-APDownload protocol
IHC protocol for NANP antibody 12519-1-APDownload protocol
IF protocol for NANP antibody 12519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols