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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NANS Polyclonal antibody

NANS Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15163-1-AP

Synonyms

N-acetylneuraminic acid phosphate synthase, N-acetylneuraminate-9-phosphate synthase, N-acetylneuraminate synthase, N acetylneuraminate synthase, EC:2.5.1.57

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NANS Polyclonal antibody (15163-1-AP)

    WB analysis of HeLa using 15163-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15163-1-AP (NANS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NANS Polyclonal antibody (15163-1-AP)

    WB analysis of human brain using 15163-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15163-1-AP (NANS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NANS Polyclonal antibody (15163-1-AP)

    WB analysis of HepG2 using 15163-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15163-1-AP (NANS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NANS Polyclonal antibody (15163-1-AP)

    IP experiment of HepG2 using 15163-1-AP

    IP result of anti-NANS (IP:15163-1-AP, 4ug; Detection:15163-1-AP 1:500) with HepG2 cells lysate 3300 ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NANS Polyclonal antibody (15163-1-AP)

    IHC staining of human stomach cancer using 15163-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15163-1-AP (NANS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NANS Polyclonal antibody (15163-1-AP)

    IHC staining of human stomach cancer using 15163-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15163-1-AP (NANS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

15163-1-AP targets NANS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NANS fusion protein Ag7271 相同性解析による交差性が予測される生物種
Full Name N-acetylneuraminic acid synthase
Calculated molecular weight 40 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC000008
Gene symbol NANS
Gene ID (NCBI) 54187
RRIDAB_2150690
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The sialic acids are a family of 9-carbon 2-keto-3-deoxy sugars. They are frequently the terminal sugars on secreted and cell surface glycoproteins and glycolipids. Sialic acids participate in many important biologic recognition events. NANS can also use N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.

Protocols

Product Specific Protocols
WB protocol for NANS antibody 15163-1-APDownload protocol
IHC protocol for NANS antibody 15163-1-APDownload protocol
IP protocol for NANS antibody 15163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Exp Cell Res

Reanalysis of single-cell data reveals macrophage subsets associated with the immunotherapy response and prognosis of patients with endometrial cancer

Authors - Qianhua Wu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

PK (Verified Customer) (03-22-2022)

  • Applications: Western Blot
  • Cell Tissue Type: LUNG Fibroblast
NANS Antibody Western Blot validation ( dilution) in LUNG Fibroblast (Cat no:15163-1-AP)