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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAP1;NCKAP1 Recombinant antibody (85142-2-RR)

    WB analysis using 85142-2-RR (same clone as 85142-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85142-2-RR (NAP1;NCKAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85142-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01841-2

    Sandwich ELISA standard curve of MP01841-2

    Sandwich ELISA standard curve of MP01841-2, Humanc NCKAP1 Recombinant Matched Antibody Pair - PBS only. 85142-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2788. 85142-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL

  • Sample test of MP01841-2

    Sample test of MP01841-2

    The mean NCKAP1 concentration was determined to be 38.80 ng/mL in HepG2 cell extract based on a 1.0 mg/mL extract load and 46.44 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP01841-2

    Cytometric bead array standard curve of MP01841-2

    Cytometric bead array standard curve of MP01841-2, NCKAP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85142-3-PBS. Detection antibody: 85142-2-PBS. Standard: Ag2788. Range: 1.563-100 ng/mL.

  • Cytometric bead array standard curve of MP01841-1

    Cytometric bead array standard curve of MP01841-1

    Cytometric bead array standard curve of MP01841-1, NCKAP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85142-1-PBS. Detection antibody: 85142-2-PBS. Standard: Ag2788. Range: 1.563-100 ng/mL.

  • Affinity and Kinetic Characterization of 85142-2-RR

    Affinity and Kinetic Characterization of 85142-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85142-2-RR against Human NAP1;NCKAP1 were performed. The affinity constant is 0.116 nM.

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NAP1;NCKAP1 Recombinant antibody, PBS Only (Detector)

NAP1;NCKAP1 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85142-2-PBS
Clone No.242784C5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

NCKAP1, NAP1, NAP 1, Membrane-associated protein HEM-2, KIAA0587

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85142-2-PBS targets NAP1;NCKAP1 as part of a matched antibody pair:

MP01841-1: 85142-1-PBS capture and 85142-2-PBS detection (validated in Cytometric bead array)

MP01841-2: 85142-3-PBS capture and 85142-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NAP1;NCKAP1 fusion protein Ag2788 相同性解析による交差性が予測される生物種
Full Name NCK-associated protein 1
Calculated molecular weight 129 kDa
Observed molecular weight 115-125 kDa
GenBank accession numberBC015025
Gene Symbol NCKAP1
Gene ID (NCBI) 10787
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2A7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NCK-associated protein 1 (NCKAP1), also known as NAP1 or HEM2, is encoded by an apoptosis-related gene human NAP1, the expression of which is strongly down-regulated in sporadic Alzheimer's disease (AD). Human NAP1 is predominantly expressed in neuronal cells. Antisense oligo DNA of human NAP1 transcripts was found to induce apoptosis of neuronal cells. Loss of NAP1 function disrupts neuronal differentiation. Likewise, NAP1 plays an essential role in facilitating neuronal cytoskeletal changes underlying the postmigratory differentiation of cortical neurons, a critical step in functional wiring of the cortex. Human NAP1 is also proved to be an orthologue of rat Nap1 which binds to the adaptor molecule NCK in signal transduction.