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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAP1L4 Polyclonal antibody (27889-1-AP)

    WB analysis using 27889-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27889-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NAP1L4 Polyclonal antibody (27889-1-AP)

    WB analysis of HeLa using 27889-1-AP

    WB result of NAP1L4 antibody (27889-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAP1L4 transfected HeLa cells.

  • Western Blot (WB) analysis of human testis tissue using NAP1L4 Polyclonal antibody (27889-1-AP)

    WB analysis of human testis using 27889-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 27889-1-AP (NAP1L4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  • KD/KO Validated

NAP1L4 Polyclonal antibody

NAP1L4 Polyclonal Antibody for WB, ELISA
Cat No. 27889-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

hNAP2, NAP 2, NAP1L4, NAP2, NAP2L, Nucleosome assembly protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HuH-7 cells, mouse heart tissue, mouse testis tissue, rat heart tissue, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27889-1-AP targets NAP1L4 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAP1L4 fusion protein Ag27469 相同性解析による交差性が予測される生物種
Full Name nucleosome assembly protein 1-like 4
Calculated molecular weight 375 aa, 43 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC022090
Gene Symbol NAP1L4
Gene ID (NCBI) 4676
RRIDAB_2881006
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99733
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NAP1L4 is a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer.

Protocols

Product Specific Protocols
WB protocol for NAP1L4 antibody 27889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols