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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha SNAP Polyclonal antibody

Alpha SNAP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10546-1-AP

Synonyms

alpha-SNAP, NAPA, SNAP alpha, SNAPA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alpha SNAP Polyclonal antibody (10546-1-AP)

    WB analysis using 10546-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10546-1-AP (alpha-SNAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Alpha SNAP Polyclonal antibody (10546-1-AP)

    WB analysis using 10546-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10546-1-AP (Alpha SNAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Alpha SNAP Polyclonal antibody (10546-1-AP)

    IHC staining of mouse brain using 10546-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10546-1-AP (Alpha SNAP antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Alpha SNAP Polyclonal antibody (10546-1-AP)

    IHC staining of mouse brain using 10546-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10546-1-AP (Alpha SNAP antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10546-1-AP targets Alpha SNAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha SNAP fusion protein Ag0836 相同性解析による交差性が予測される生物種
Full Name N-ethylmaleimide-sensitive factor attachment protein, alpha
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC007432
Gene symbol Alpha SNAP
Gene ID (NCBI) 8775
RRIDAB_2150721
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNAPs (soluble NSF-attachment proteins) may play an important role in the process of docking and fusion of vesicles to their target membranes, according to the 'SNARE hypothesis'. NAPA(Alpha- SNAP) was found in a wide range of tissues and act synergistically in intra-Golgi transport. This antibody recognizes a 33kd protein in SDS page.

Protocols

Product Specific Protocols
WB protocol for Alpha SNAP antibody 10546-1-APDownload protocol
IHC protocol for Alpha SNAP antibody 10546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols