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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAPG Polyclonal antibody (15604-1-AP)

    WB analysis using 15604-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15604-1-AP (NAPG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NAPG Polyclonal antibody (15604-1-AP)

    WB analysis of HeLa using 15604-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15604-1-AP (NAPG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NAPG Polyclonal antibody (15604-1-AP)

    WB analysis of HepG2 using 15604-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15604-1-AP (NAPG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using NAPG Polyclonal antibody (15604-1-AP)

    WB analysis of K-562 using 15604-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15604-1-AP (NAPG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NAPG Polyclonal antibody (15604-1-AP)

    IP experiment of HepG2 using 15604-1-AP

    IP result of anti-NAPG (IP:15604-1-AP, 4ug; Detection:15604-1-AP 1:400) with HepG2 cells lysate 3300 ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NAPG Polyclonal antibody (15604-1-AP)

    IHC staining of human ovary tumor using 15604-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15604-1-AP (NAPG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NAPG Polyclonal antibody (15604-1-AP)

    IHC staining of human ovary tumor using 15604-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15604-1-AP (NAPG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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NAPG Polyclonal antibody

NAPG Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15604-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

SNAP-gamma, SNAPG, SNAP gamma, N-ethylmaleimide-sensitive factor attachment protein gamma, Gamma-soluble NSF attachment protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells, K-562 cells, HepG2 cells, HeLa cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15604-1-AP targets NAPG in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAPG fusion protein Ag7988 相同性解析による交差性が予測される生物種
Full Name N-ethylmaleimide-sensitive factor attachment protein, gamma
Calculated molecular weight 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC001889
Gene Symbol NAPG
Gene ID (NCBI) 8774
RRIDAB_2150885
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99747
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NAPG antibody 15604-1-APDownload protocol
IHC protocol for NAPG antibody 15604-1-APDownload protocol
IP protocol for NAPG antibody 15604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols