NAPRT1 Polyclonal antibody

Name: NAPRT1 Polyclonal antibody
Brand: Proteintech
SKU: 13549-1-AP

NAPRT1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13549-1-AP

Synonyms

FHA HIT interacting protein, FHIP, NAPRT1, NAPRTase, PP3856

Validation Data Gallery

Filter:

AllWBIP

WB analysis using 13549-1-AP
Mouse liver tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 13549-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 13549-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13549-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 13549-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13549-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human small intestine using 13549-1-AP
human small intestine tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 13549-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 13549-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13549-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 13549-1-AP
IP result of anti-NAPRT1 (IP:13549-1-AP, 4ug; Detection:13549-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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Cat No. 13549-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, human colon tissue, HEK-293 cells, mouse kidney tissue, L02 cells, HepG2 cells
Positive IP detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 15 publications below
IHC	See 4 publications below
IF	See 3 publications below

Product Information

13549-1-AP targets NAPRT1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NAPRT1 fusion protein Ag4265 相同性解析による交差性が予測される生物種
Full Name	nicotinate phosphoribosyltransferase domain containing 1
Calculated molecular weight	514 aa, 55 kDa
Observed molecular weight	51 kDa, 56 kDa
GenBank accession number	BC032466
Gene symbol	NAPRT1
Gene ID (NCBI)	93100
RRID	AB_2151014
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).

Protocols

Product Specific Protocols
WB protocol for NAPRT1 antibody 13549-1-AP	Download protocol
IP protocol for NAPRT1 antibody 13549-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Cell Metab NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion. Authors - Hongwei Lv View Article
human	IF,WB	J Pharm Anal Epigenetic drug library screening reveals targeting DOT1L abrogates NAD+ synthesis by reprogramming H3K79 methylation in uveal melanoma Authors - Xiang Gu View Article
	WB	Kidney Int Anticystogenic activity of a small molecule PAK4 inhibitor may be a novel treatment for autosomal dominant polycystic kidney disease. Authors - Vicki J Hwang View Article
human	WB	Clin Cancer Res Targeting of NAD metabolism in pancreatic cancer cells: potential novel therapy for pancreatic tumors. Authors - Chini Claudia Cs CC View Article
human,mouse	WB,IHC	Int J Mol Sci NAD+ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy. Authors - Xue Li View Article
human,mouse	IF	Metabolism Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice. Authors - Ya Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NAPRT1 Polyclonal antibody

NAPRT1 Polyclonal Antibody for WB, IP, ELISA

Validation Data Gallery

WB analysis using 13549-1-AP

WB analysis of L02 using 13549-1-AP

WB analysis of L02 using 13549-1-AP

WB analysis of HepG2 using 13549-1-AP

WB analysis of mouse liver using 13549-1-AP

WB analysis of HEK-293 using 13549-1-AP

WB analysis of human small intestine using 13549-1-AP

WB analysis of mouse kidney using 13549-1-AP

WB analysis of human colon using 13549-1-AP

WB analysis of HEK-293 using 13549-1-AP

IP experiment of HEK-293 using 13549-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion.

Epigenetic drug library screening reveals targeting DOT1L abrogates NAD+ synthesis by reprogramming H3K79 methylation in uveal melanoma

Anticystogenic activity of a small molecule PAK4 inhibitor may be a novel treatment for autosomal dominant polycystic kidney disease.

Targeting of NAD metabolism in pancreatic cancer cells: potential novel therapy for pancreatic tumors.

NAD+ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy.

Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice.