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Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using NARF Polyclonal antibody (12173-1-AP)

    WB analysis of human liver using 12173-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using NARF Polyclonal antibody (12173-1-AP)

    WB analysis of human placenta using 12173-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using NARF Polyclonal antibody (12173-1-AP)

    WB analysis of mouse testis using 12173-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lung tissue using NARF Polyclonal antibody (12173-1-AP)

    WB analysis of human lung using 12173-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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NARF Polyclonal antibody

NARF Polyclonal Antibody for WB, ELISA
Cat No. 12173-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

IOP2, NARF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman liver tissue, human lung tissue, human placenta tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12173-1-AP targets NARF in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NARF fusion protein Ag2816 相同性解析による交差性が予測される生物種
Full Name nuclear prelamin A recognition factor
Calculated molecular weight 46 kDa, 53 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC016440
Gene Symbol NARF
Gene ID (NCBI) 26502
RRIDAB_2151027
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHQ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Prelamin A, is processed in the nucleus to lamin A by removal of its final 18 amino acids, including the cysteine residue in its C-terminal CAAX box, which is farnesylated. Nuclear prelamin A recognition factor (NARF) binds the farnesylated prelamin A C-terminal domain(preAct)(PMID:10514485). NARF, at 52 kDa, also named as IOP2, is a small enough protein to freely diffuse through the nuclear pore complex and may be retained within the nucleus by binding nuclear proteins and it has all of the information necessary to be imported into the nucleus(PMID:10514485). It has 3 isoforms produced by alternative splicing with the molecular mass of 51 kDa, 56 kDa and 46 kDa,respectively.

Protocols

Product Specific Protocols
WB protocol for NARF antibody 12173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols