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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NARFL Polyclonal antibody (13652-1-AP)

    WB analysis using 13652-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13652-1-AP (NARFL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using NARFL Polyclonal antibody (13652-1-AP)

    IHC staining of human colon using 13652-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13652-1-AP (NARFL antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using NARFL Polyclonal antibody (13652-1-AP)

    IHC staining of human colon using 13652-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13652-1-AP (NARFL antibody) at dilution of 1:200 (under 40x lens).

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NARFL Polyclonal antibody

NARFL Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13652-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

HPRN, IOP1, LET1L, NARFL, PRN, Protein related to Narf

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

13652-1-AP targets NARFL in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NARFL fusion protein Ag4217 相同性解析による交差性が予測される生物種
Full Name nuclear prelamin A recognition factor-like
Calculated molecular weight 476 aa, 53 kDa
Observed molecular weight 26-28 kDa
GenBank accession numberBC030248
Gene Symbol NARFL
Gene ID (NCBI) 64428
RRIDAB_2877965
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H6Q4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NARFL(Nuclear prelamin A recognition factor-like protein) is also named as PRN and belongs to the NARF family. This protein plays a role in cytoplasmic Fe-S protein biogenesis in mammalian cells. NARFL has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for NARFL antibody 13652-1-APDownload protocol
IHC protocol for NARFL antibody 13652-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Rep

NARFL deficiency caused mitochondrial dysfunction in lung cancer cells by HIF-1α-DNMT1 axis

Authors - Hongzhou Liu
View Article