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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using NARFL-Specific Polyclonal antibody (20209-1-AP)

    WB analysis of HEK-293 using 20209-1-AP (same clone as 20209-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20209-1-AP (NARFL-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20209-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NARFL-Specific Polyclonal antibody (20209-1-AP)

    WB analysis using 20209-1-AP (same clone as 20209-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20209-1-AP (NARFL-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20209-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NARFL-Specific Polyclonal antibody (20209-1-AP)

    WB analysis of HEK-293 using 20209-1-AP (same clone as 20209-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20209-1-AP (NARFL-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NARFL-Specific Polyclonal antibody (20209-1-AP)

    IHC staining of human kidney using 20209-1-AP (same clone as 20209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NARFL-Specific Polyclonal antibody (20209-1-AP)

    IHC staining of human kidney using 20209-1-AP (same clone as 20209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using NARFL-Specific Polyclonal antibody (20209-1-AP)

    IHC staining of human heart using 20209-1-AP (same clone as 20209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using NARFL-Specific Polyclonal antibody (20209-1-AP)

    IHC staining of human heart using 20209-1-AP (same clone as 20209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20209-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20209-1-PBS targets NARFL-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name nuclear prelamin A recognition factor-like
Calculated molecular weight 53 kDa
Observed molecular weight 26-28 kDa
GenBank accession numberNM_022493
Gene Symbol NARFL
Gene ID (NCBI) 64428
RRIDAB_10694435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H6Q4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NARFL, also named as PRN and IOP1, belongs to the NARF family. NAFL is a component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. It is required for maturation of extramitochondrial Fe/S proteins. It seems to negatively regulate the level of HIF1A expression, although this effect could be indirect. [18270200], [16956324] The antibody is specific to NARFL.