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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NARG1 Polyclonal antibody (23845-1-AP)

    WB analysis of HeLa using 23845-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23845-1-AP (NARG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NARG1 Polyclonal antibody (23845-1-AP)

    WB analysis of HEK-293 using 23845-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23845-1-AP (NARG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NARG1 Polyclonal antibody (23845-1-AP)

    IHC staining of mouse kidney using 23845-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23845-1-AP (NARG1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NARG1 Polyclonal antibody (23845-1-AP)

    IHC staining of human stomach cancer using 23845-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23845-1-AP (NARG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NARG1 Polyclonal antibody (23845-1-AP)

    IF Staining of HeLa using 23845-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 23845-1-AP (NARG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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NARG1 Polyclonal antibody

NARG1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23845-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NMDA receptor-regulated protein 1, N-alpha-acetyltransferase 15, NatA auxiliary subunit, NAA15, N terminal acetyltransferase, Gastric cancer antigen Ga19

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman stomach cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23845-1-AP targets NARG1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NARG1 fusion protein Ag20842 相同性解析による交差性が予測される生物種
Full Name NMDA receptor regulated 1
Calculated molecular weight 866 aa, 101 kDa
Observed molecular weight 95-100 kDa
GenBank accession numberBC093928
Gene Symbol NARG1
Gene ID (NCBI) 80155
RRIDAB_2879338
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9BXJ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NAA15 (N-alpha-acetyltransferase 15) is a subunit of both the NatA and NatE complexes, and its role is to position the catalytic subunits in close vicinity to the nascent polypeptides (PMID: 33557580). Human NAA15 encodes an 866 amino acid (~105 kDa) protein, NAA15, containing tetratricopeptide repeat domains and a putative bipartite nuclear localization signal (PMID: 29656860). NAA15 was implicated in human disease and loss-of-function NAA15 variants in large cohorts of patients with autism spectrum disorder and/or ID (PMID: 33103328).

Protocols

Product Specific Protocols
WB protocol for NARG1 antibody 23845-1-APDownload protocol
IHC protocol for NARG1 antibody 23845-1-APDownload protocol
IF protocol for NARG1 antibody 23845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols