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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NARS Monoclonal antibody (67711-1-Ig)

    WB analysis using 67711-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67711-1-Ig (NARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using NARS Monoclonal antibody (67711-1-Ig)

    IHC staining of human urothelial carcinoma using 67711-1-Ig

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67711-1-Ig (NARS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NARS Monoclonal antibody (67711-1-Ig)

    IF Staining of HeLa using 67711-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NARS antibody (67711-1-Ig, Clone: 4G5G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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NARS Monoclonal antibody

NARS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67711-1-Ig
Clone No.4G5G12

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ASNRS, Asparagine tRNA ligase, asparaginyl tRNA synthetase, NARS, NARS1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67711-1-Ig targets NARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NARS fusion protein Ag6656 相同性解析による交差性が予測される生物種
Full Name asparaginyl-tRNA synthetase
Calculated molecular weight 63 kDa
Observed molecular weight63 kDa
GenBank accession numberBC001687
Gene Symbol NARS
Gene ID (NCBI) 4677
RRIDAB_2882901
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43776
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Asparagine-tRNA ligase (NARS) belongs to the class-II aminoacyl-tRNA synthetase family. Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (PubMed: 9421509). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells. The NARS levels were higher in tumor tissues compared with adjacent normal tissues, and positively associated with LN metastasis. NARS may serve as a potential biomarker for lung adenocarcinoma (ADC) diagnosis/prognosis (PMID: 27161446).

Protocols

Product Specific Protocols
WB protocol for NARS antibody 67711-1-IgDownload protocol
IHC protocol for NARS antibody 67711-1-IgDownload protocol
IF protocol for NARS antibody 67711-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols