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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NARS2 Polyclonal antibody (15714-1-AP)

    WB analysis using 15714-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15714-1-AP (NARS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NARS2 Polyclonal antibody (15714-1-AP)

    WB analysis of HepG2 using 15714-1-AP

    WB result of NARS2 antibody (15714-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NARS2 transfected HepG2 cells.

  • Western Blot (WB) analysis of human liver tissue using NARS2 Polyclonal antibody (15714-1-AP)

    WB analysis of human liver using 15714-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15714-1-AP (NARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NARS2 Polyclonal antibody (15714-1-AP)

    WB analysis of human brain using 15714-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15714-1-AP (NARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NARS2 Polyclonal antibody (15714-1-AP)

    IHC staining of human kidney using 15714-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15714-1-AP (NARS2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NARS2 Polyclonal antibody (15714-1-AP)

    IHC staining of human kidney using 15714-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15714-1-AP (NARS2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using NARS2 Polyclonal antibody (15714-1-AP)

    IHC staining of human heart using 15714-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using NARS2 Polyclonal antibody (15714-1-AP)

    IHC staining of human heart using 15714-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using NARS2 Polyclonal antibody (15714-1-AP)

    IHC staining of human placenta using 15714-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using NARS2 Polyclonal antibody (15714-1-AP)

    IHC staining of human placenta using 15714-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using NARS2 Polyclonal antibody (15714-1-AP)

    IHC staining of human spleen using 15714-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using NARS2 Polyclonal antibody (15714-1-AP)

    IHC staining of human spleen using 15714-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using NARS2 Polyclonal antibody (15714-1-AP)

    IHC staining of human lung using 15714-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using NARS2 Polyclonal antibody (15714-1-AP)

    IHC staining of human lung using 15714-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NARS2 Polyclonal antibody (15714-1-AP)

    IF Staining of HepG2 using 15714-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15714-1-AP (NARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NARS2 Polyclonal antibody (15714-1-AP)

    IF Staining of HepG2 using 15714-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15714-1-AP (NARS2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

NARS2 Polyclonal antibody

NARS2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15714-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AsnRS, Asparagine tRNA ligase, NARS2, SLM5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, human liver tissue, human brain tissue, K-562 cells, mouse kidney tissue
Positive IHC detected inhuman kidney tissue, human heart tissue, human lung tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15714-1-AP targets NARS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NARS2 fusion protein Ag8345 相同性解析による交差性が予測される生物種
Full Name asparaginyl-tRNA synthetase 2, mitochondrial (putative)
Calculated molecular weight 477 aa, 54 kDa
Observed molecular weight 50-54 kDa
GenBank accession numberBC007800
Gene Symbol NARS2
Gene ID (NCBI) 79731
RRIDAB_2151157
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96I59
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NARS2 antibody 15714-1-APDownload protocol
IHC protocol for NARS2 antibody 15714-1-APDownload protocol
IF protocol for NARS2 antibody 15714-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols