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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using NAS1/SLC13A1 Polyclonal antibody (25039-1-AP)

    WB analysis of HEK-293 using 25039-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25039-1-AP (NAS1/SLC13A1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using NAS1/SLC13A1 Polyclonal antibody (25039-1-AP)

    WB analysis of mouse kidney using 25039-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25039-1-AP (NAS1/SLC13A1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using NAS1/SLC13A1 Polyclonal antibody (25039-1-AP)

    IF Staining of mouse kidney using 25039-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NAS1/SLC13A1 antibody (25039-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using NAS1/SLC13A1 Polyclonal antibody (25039-1-AP)

    IF Staining of mouse kidney using 25039-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NAS1/SLC13A1 antibody (25039-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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NAS1/SLC13A1 Polyclonal antibody

NAS1/SLC13A1 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 25039-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

SLC13A1, NAS1, hNaSi-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25039-1-AP targets NAS1/SLC13A1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAS1/SLC13A1 fusion protein Ag19026 相同性解析による交差性が予測される生物種
Full Name solute carrier family 13 (sodium/sulfate symporters), member 1
Calculated molecular weight 595 aa, 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC148686
Gene Symbol NAS1
Gene ID (NCBI) 6561
RRIDAB_2879864
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZW2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC13A1 also known as NAS1, is an apical membrane Na(+)-sulfate cotransporter involved in sulfate homeostasis in the kidney. SLC13A1 primarily participates in pH-independent, sodium-dependent cotransport of sulfate across renal proximal tubules and small intestinal epithelia(PMID: 38552027).

Protocols

Product Specific Protocols
WB protocol for NAS1/SLC13A1 antibody 25039-1-APDownload protocol
IF protocol for NAS1/SLC13A1 antibody 25039-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols