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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using NAT1 Polyclonal antibody (14394-1-AP)

    WB analysis of A375 using 14394-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 14394-1-AP (NAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using NAT1 Polyclonal antibody (14394-1-AP)

    WB analysis of PC-3 using 14394-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14394-1-AP (NAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NAT1 Polyclonal antibody (14394-1-AP)

    IHC staining of human breast cancer using 14394-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14394-1-AP (NAT1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NAT1 Polyclonal antibody (14394-1-AP)

    IHC staining of human breast cancer using 14394-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14394-1-AP (NAT1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using NAT1 Polyclonal antibody (14394-1-AP)

    IF Staining of A375 using 14394-1-AP

    Immunofluorescent analysis of A375 cells using 14394-1-AP (NAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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NAT1 Polyclonal antibody

NAT1 Polyclonal Antibody for WB, IF, IHC, ELISA
Cat No. 14394-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

AAC1, Arylamide acetylase 1, MNAT, N acetyltransferase type 1, NAT 1, NAT1, NATI

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14394-1-AP targets NAT1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAT1 fusion protein Ag5885 相同性解析による交差性が予測される生物種
Full Name N-acetyltransferase 1 (arylamine N-acetyltransferase)
Calculated molecular weight 34 kDa
Observed molecular weight 32-34 kDa
GenBank accession numberBC047666
Gene Symbol NAT1
Gene ID (NCBI) 9
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18440
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.