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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NAA40 Polyclonal antibody

NAA40 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16698-1-AP

Synonyms

NAT11, N-alpha-acetyltransferase 40, N-acetyltransferase 11, N alpha acetyltransferase 40, NatD catalytic subunit, N acetyltransferase 11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAA40 Polyclonal antibody (16698-1-AP)

    WB analysis using 16698-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16698-1-AP (NAT11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using NAA40 Polyclonal antibody (16698-1-AP)

    IHC staining of human ovarian cancer using 16698-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16698-1-AP (NAA40 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NAA40 Polyclonal antibody (16698-1-AP)

    IF Staining of U2OS using 16698-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NAA40 antibody (16698-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, mouse spleen tissue
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16698-1-AP targets NAA40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAA40 fusion protein Ag10092 相同性解析による交差性が予測される生物種
Full Name N-acetyltransferase 11 (GCN5-related, putative)
Calculated molecular weight 237 aa, 27 kDa
Observed molecular weight27 kDa
GenBank accession numberBC052298
Gene symbol NAA40
Gene ID (NCBI) 79829
RRIDAB_3085505
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

N-alpha-acetyltransferase 40 (NAA40, also known as NatD, Nat4, or Patt1) is a highly specific epigenetic enzyme catalyzing the transfer of an acetyl moiety at the N-terminal end of histones H4 and H2A (PMID: 26666750). NAA40 is significantly upregulated in primary CRC tissues and promotes CRC cell growth both in vitro and in xenograft tumor models (PMID: 30858358). Western blot analysis detected NAA40 at an apparent molecular mass of 27 kDa.

Protocols

Product Specific Protocols
WB protocol for NAA40 antibody 16698-1-APDownload protocol
IHC protocol for NAA40 antibody 16698-1-APDownload protocol
IF protocol for NAA40 antibody 16698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols