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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NAA40 Polyclonal antibody

NAA40 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16698-1-AP

Synonyms

NAT11, N-alpha-acetyltransferase 40, N-acetyltransferase 11, N alpha acetyltransferase 40, NatD catalytic subunit, N acetyltransferase 11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAA40 Polyclonal antibody (16698-1-AP)

    WB analysis using 16698-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16698-1-AP (NAT11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using NAA40 Polyclonal antibody (16698-1-AP)

    IHC staining of human ovarian cancer using 16698-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16698-1-AP (NAA40 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NAA40 Polyclonal antibody (16698-1-AP)

    IF Staining of U2OS using 16698-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NAA40 antibody (16698-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, mouse spleen tissue
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16698-1-AP targets NAA40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAA40 fusion protein Ag10092 相同性解析による交差性が予測される生物種
Full Name N-acetyltransferase 11 (GCN5-related, putative)
Calculated molecular weight 237 aa, 27 kDa
Observed molecular weight27 kDa
GenBank accession numberBC052298
Gene symbol NAA40
Gene ID (NCBI) 79829
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

N-alpha-acetyltransferase 40 (NAA40, also known as NatD, Nat4, or Patt1) is a highly specific epigenetic enzyme catalyzing the transfer of an acetyl moiety at the N-terminal end of histones H4 and H2A (PMID: 26666750). NAA40 is significantly upregulated in primary CRC tissues and promotes CRC cell growth both in vitro and in xenograft tumor models (PMID: 30858358). Western blot analysis detected NAA40 at an apparent molecular mass of 27 kDa.

Protocols

Product Specific Protocols
WB protocol for NAA40 antibody 16698-1-APDownload protocol
IHC protocol for NAA40 antibody 16698-1-APDownload protocol
IF protocol for NAA40 antibody 16698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols