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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAT2 Polyclonal antibody (11410-1-AP)

    WB analysis using 11410-1-AP (same clone as 11410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11410-1-AP (NAT2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11410-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using NAT2 Polyclonal antibody (11410-1-AP)

    IHC staining of human small intestine using 11410-1-AP (same clone as 11410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11410-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NAT2 Polyclonal antibody (11410-1-AP)

    IHC staining of human breast cancer using 11410-1-AP (same clone as 11410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11410-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NAT2 Polyclonal antibody (11410-1-AP)

    IHC staining of human breast cancer using 11410-1-AP (same clone as 11410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11410-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NAT2 Polyclonal antibody (11410-1-AP)

    IHC staining of human breast cancer using 11410-1-AP (same clone as 11410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11410-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using NAT2 Polyclonal antibody (11410-1-AP)

    FC experiment of MCF-7 using 11410-1-AP (same clone as 11410-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug NAT2 Polyclonal antibody (11410-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11410-1-PBS in a different storage buffer formulation.

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NAT2 Polyclonal antibody, PBS Only

NAT2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11410-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11410-1-PBS targets NAT2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAT2 fusion protein Ag1953 相同性解析による交差性が予測される生物種
Full Name N-acetyltransferase 2 (arylamine N-acetyltransferase)
Calculated molecular weight 33.5 kDa
Observed molecular weight38 kDa
GenBank accession numberBC015878
Gene Symbol NAT2
Gene ID (NCBI) 10
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11245
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NAT2 (Arylamine N-acetyltransferase 2) is well-known for its role in the phase II metabolism of xenobiotics and drugs (PMID:36156098). NAT2 and its isozyme, arylamine N-acetyltransferase 1 (NAT1), play key roles in the detoxification of carcinogenic arylamines and xenobiotics by catalyzing acetyl-coenzyme A (CoA)-dependent biotransformation of arylamines to N-arylacetamides. Endogenous NAT2 expression is limited to the liver and small and large intestines, suggesting that NAT2 plays organ-specific roles (PMID:24467436).