• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using NAT5 Polyclonal antibody (15807-1-AP)

    WB analysis of HeLa using 15807-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15807-1-AP (NAT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NAT5 Polyclonal antibody (15807-1-AP)

    WB analysis of human brain using 15807-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15807-1-AP (NAT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using NAT5 Polyclonal antibody (15807-1-AP)

    WB analysis of HL-60 using 15807-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 15807-1-AP (NAT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HL-60 cells using NAT5 Polyclonal antibody (15807-1-AP)

    IP experiment of HL-60 using 15807-1-AP

    IP result of anti-NAT5 (IP:15807-1-AP, 4ug; Detection:15807-1-AP 1:500) with HL-60 cells lysate 3040ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using NAT5 Polyclonal antibody (15807-1-AP)

    IHC staining of human ovary cancer using 15807-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15807-1-AP (NAT5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NAT5 Polyclonal antibody (15807-1-AP)

    IF Staining of HeLa using 15807-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15807-1-AP (NAT5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

NAT5 Polyclonal antibody

NAT5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15807-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

N-alpha-acetyltransferase 20, N-acetyltransferase 5, NAA20, N acetyltransferase 5, Methionine N-acetyltransferase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HL-60 cells, human brain tissue
Positive IP detected inHL-60 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15807-1-AP targets NAT5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAT5 fusion protein Ag8541 相同性解析による交差性が予測される生物種
Full Name N-acetyltransferase 5 (GCN5-related, putative)
Calculated molecular weight 178 aa, 20 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC005181
Gene Symbol NAT5
Gene ID (NCBI) 51126
RRIDAB_1640056
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61599
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NAT5 antibody 15807-1-APDownload protocol
IHC protocol for NAT5 antibody 15807-1-APDownload protocol
IF protocol for NAT5 antibody 15807-1-APDownload protocol
IP protocol for NAT5 antibody 15807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

NatB Catalytic Subunit Depletion Disrupts DNA Replication Initiation Leading to Senescence in MEFs

Authors - Jasmin Elurbide
View Article
humanWB

Oncotarget

NatB-mediated protein N-α-terminal acetylation is a potential therapeutic target in hepatocellular carcinoma.

Authors - Leire Neri
View Article
mouse

Mol Cell Biol

NatB Protects Procaspase-8 from UBR4-Mediated Degradation and Is Required for Full Induction of the Extrinsic Apoptosis Pathway

Authors - Joana P Guedes
View Article