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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAT6/FUS2 Polyclonal antibody (15476-1-AP)

    WB analysis using 15476-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15476-1-AP (NAT6/FUS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NAT6/FUS2 Polyclonal antibody (15476-1-AP)

    IF Staining of HeLa using 15476-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAT6/FUS2 antibody (15476-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

NAT6/FUS2 Polyclonal antibody

NAT6/FUS2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15476-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

NAA80, NAT6, N-acetyltransferase 6, HsNAAA80, FUS2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15476-1-AP targets NAT6/FUS2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAT6/FUS2 fusion protein Ag7780 相同性解析による交差性が予測される生物種
Full Name N-acetyltransferase 6 (GCN5-related)
Calculated molecular weight 31 kDa
Observed molecular weight45 kDa
GenBank accession numberBC004483
Gene Symbol FUS2
Gene ID (NCBI) 24142
RRIDAB_3085463
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ93015
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The acetyltransferase NAT6 (also known as Fus2) is an enzyme that catalyzes the transfer of acetyl groups from acetyl-CoA to acrylamines and located mainly in the cytoplasm. NAT6 has two isoforms. The molecular weight of NAT6 was detected to be about 45kDa (PMID:32978259).

Protocols

Product Specific Protocols
WB protocol for NAT6/FUS2 antibody 15476-1-APDownload protocol
IF protocol for NAT6/FUS2 antibody 15476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

FEBS J

NAT6 acetylates the N-terminus of different forms of actin.

Authors - Elsa Wiame
  • KO Validated
View Article
humanWB

Brain Commun

NAA80 bi-allelic missense variants result in high-frequency hearing loss, muscle weakness and developmental delay.

Authors - Irena J J Muffels
View Article