• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NAT8L Polyclonal antibody

NAT8L Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23841-1-AP

Synonyms

NAA synthetase, Hcml3, EC:2.3.1.17, CML3, Camello-like protein 3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NAT8L Polyclonal antibody (23841-1-AP)

    WB analysis using 23841-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23841-1-AP (NAT8L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using NAT8L Polyclonal antibody (23841-1-AP)

    IF Staining of HEK-293 using 23841-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NAT8L antibody (23841-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

☆ 2/28（金）まで！最大30%オフ！ >> トライアルサイズもお得な一次抗体キャンペーン

Tested Applications

Positive WB detected inHUVEC cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

23841-1-AP targets NAT8L in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAT8L fusion protein Ag20832 相同性解析による交差性が予測される生物種
Full Name N-acetyltransferase 8-like (GCN5-related, putative)
Calculated molecular weight 134 aa, 15 kDa
Observed molecular weight48 kDa
GenBank accession numberBC103748
Gene symbol NAT8L
Gene ID (NCBI) 339983
RRIDAB_3669418
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NAT8L (N-acetyltransferase 8-like) catalyzes the formation of N-acetyl aspartate (NAA) from acetyl-CoA and aspartate. In the brain, NAA delivers the acetate moiety to synthesize acetyl-CoA, which is further used for fatty acid generation (PMID: 24155240). NAT8L modulation may impinge on the metabolic reprogramming of cancer cells (PMID: 36580805). The calculated MW of NAT8L is around 33 kDa, and the observed MW is around 45 kDa.

Protocols

Product Specific Protocols
WB protocol for NAT8L antibody 23841-1-APDownload protocol
IF protocol for NAT8L antibody 23841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Naunyn Schmiedebergs Arch Pharmacol

Molecular mechanism of geniposide against ANIT-induced intrahepatic cholestasis by integrative analysis of transcriptomics and metabolomics

Authors - Junyi Zhang
View Article