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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAXE Polyclonal antibody (32022-1-AP)

    WB analysis using 32022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32022-1-AP (NAXE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using NAXE Polyclonal antibody (32022-1-AP)

    IF Staining of A549 using 32022-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using NAXE antibody (32022-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

NAXE Polyclonal antibody

NAXE Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32022-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

NAD(P)H-hydrate epimerase, EC:5.1.99.6, Apolipoprotein A-I-binding protein, APOA1BP, AI-BP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, L02 cells, MCF-7 cells, NIH/3T3 cells, mouse liver tissue, rat kidney tissue
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32022-1-AP targets NAXE in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAXE fusion protein Ag35306 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-I binding protein
Calculated molecular weight32 kDa
Observed molecular weight29 kDa
GenBank accession numberNM_144772.2
Gene Symbol APOA1BP
Gene ID (NCBI) 128240
RRIDAB_3670173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8NCW5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NAD(P)H-hydrate epimerase (NAXE, also known as AIBP, YJEFN1, and APOA1BP) catalyzes the interconversion between the S- and R-forms of NAD(P)HX, and essentially supplies the substrate for the stereospecific enzyme NAD(P)HX dehydratase (NAXD) (PMID: 35866541). NAXE variants can cause neurometabolic disorder by disrupting the cellular NAD(P)HX repair system (PMID: 31745726; 27616477).

Protocols

Product Specific Protocols
WB protocol for NAXE antibody 32022-1-APDownload protocol
IF protocol for NAXE antibody 32022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols