NBCe2 Polyclonal antibody
NBCe2 Polyclonal Antibody for IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse and More (2)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 26150-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human liver cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
The immunogen of 26150-1-AP is NBCe2 Fusion Protein expressed in E. coli.
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NBCe2 fusion protein Ag23558 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 4, sodium bicarbonate cotransporter, member 5 |
| Observed molecular weight | 108-127 kDa |
| GenBank accession number | BC109221 |
| Gene symbol | SLC4A5 |
| Gene ID (NCBI) | 57835 |
| RRID | AB_2918099 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NBCe2, also named as SLC4A5 and NBC4, belongs to the anion exchanger (TC 2.A.31) family. NBCe2 mediates electrogenic Na+:HCO3 − transport, and can operate in both a 1:2, and 1:3 manner, but the transport stoichiometry as well as directionality have not been defined for the renal NBCe2. The expression of NBCe2 has been reported in nearly all kidney nephron segments and CDs, indicating that some species-specificity might be present (PMID: 32547422). NBCe2 has 8 isoforms with the molecular mass of 108-127 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NBCe2 antibody 26150-1-AP | Download protocol |
| IF protocol for NBCe2 antibody 26150-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Cell-Type Dependent Regulation of the Electrogenic Na+/HCO3- Cotransporter 1 (NBCe1) by Hypoxia and Acidosis in Glioblastoma | ||
Neuron The sodium-bicarbonate cotransporter Slc4a5 mediates feedback at the first synapse of vision |
