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NBN / NBS1 Recombinant antibody

NBN / NBS1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 82884-1-RR
Clone No.1C16

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

AT V1, AT V2, ATV, FLJ10155, NBN, NBS, NBS1, nibrin, P95

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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Tested Applications

Positive WB detected inJurkat cells, HeLa cells, A549 cells, A431 cells,

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82884-1-RR targets NBN / NBS1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name nibrin
Calculated molecular weight 85 kDa
Observed molecular weight 90-95 kDa
GenBank accession numberBC136803
Gene Symbol NBN
Gene ID (NCBI) 4683
RRIDAB_3670602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60934
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NBN, also named as NBS, NBS1, and P95, is a component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control, and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. NBN modulates the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase-dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoints and there is some evidence that NBN is involved in G1 and G2 checkpoints. Defects in NBN are the cause of Nijmegen breakage syndrome (NBS). Defects in NBN are a cause of genetic susceptibility to breast cancer (BC). Defects in NBN may be associated with aplastic anemia. Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL). The antibody is specific to NBN. The full-length NBN protein, with an apparent molecular weight of 95 kDa and the two protein fragments of 26 and 70 kDa arising from the c.657_661del5 (p.K219fsX19) mutation, and the 80 kDa protein found in patient RR with the mutation c.742_743insGG leading to excision of exons 6 and 7 from the NBN mRNA are shown. (PMID: 26265251) The predicted molecular weight of NBN protein (p95) is 85kDa, and actually detection result is about 95kDa(PMID: 23762398).

Protocols

Product Specific Protocols
WB protocol for NBN / NBS1 antibody 82884-1-RRDownload protocol
FC protocol for NBN / NBS1 antibody 82884-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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