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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NCALD Monoclonal antibody

NCALD Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6B4E10

Cat no : 66088-1-Ig

Synonyms

NCALD, neurocalcin delta, neurocalcin δ

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCALD Monoclonal antibody (66088-1-Ig)

    WB analysis using 66088-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NCALD Monoclonal antibody (66088-1-Ig)

    WB analysis using 66088-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NCALD Monoclonal antibody (66088-1-Ig)

    WB analysis using 66088-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NCALD Monoclonal antibody (66088-1-Ig)

    WB analysis of human brain using 66088-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of human cerebellum using 66088-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 66088-1-Ig(NCALD antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of human testis using 66088-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66088-1-Ig (NCALD Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of human testis using 66088-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66088-1-Ig (NCALD Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of mouse cerebellum using 66088-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of mouse cerebellum using 66088-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of human cerebellum using 66088-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 66088-1-Ig(NCALD antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of mouse brain using 66088-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of mouse brain using 66088-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of mouse cerebellum using 66088-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of mouse cerebellum using 66088-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NCALD Monoclonal antibody (66088-1-Ig)

    IF Staining of HeLa using 66088-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66088-1-Ig (NCALD antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected infetal human brain tissue, human brain tissue, pig cerebellum tissue, pig brain tissue, rat brain tissue, mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IHC detected inhuman cerebellum tissue, mouse cerebellum tissue, human testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3200
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66088-1-Ig targets NCALD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NCALD fusion protein Ag18760 相同性解析による交差性が予測される生物種
Full Name neurocalcin delta
Calculated molecular weight 193 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC036098
Gene symbol NCALD
Gene ID (NCBI) 83988
RRIDAB_11232229
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.

Protocols

Product Specific Protocols
WB protocol for NCALD antibody 66088-1-IgDownload protocol
IHC protocol for NCALD antibody 66088-1-IgDownload protocol
IF protocol for NCALD antibody 66088-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols