Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, Neuro-2a cells, rat brain tissue, pig brain tissue |
Positive IHC detected in | human lung cancer tissue, human appendicitis tissue, human colon tissue, human tonsillitis tissue, Insulinoma tissue, mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:20000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 14 publications below |
IHC | See 35 publications below |
IF | See 21 publications below |
Product Information
14255-1-AP targets NCAM1/CD56 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NCAM1/CD56 fusion protein Ag5528 相同性解析による交差性が予測される生物種 |
Full Name | neural cell adhesion molecule 1 |
Calculated molecular weight | 95 kDa |
Observed molecular weight | 120 kDa, 140 kDa, 180 kDa |
GenBank accession number | BC047244 |
Gene Symbol | NCAM1 |
Gene ID (NCBI) | 4684 |
ENSEMBL Gene ID | ENSG00000149294 |
RRID | AB_2149421 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P13591 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for NCAM1/CD56 antibody 14255-1-AP | Download protocol |
IHC protocol for NCAM1/CD56 antibody 14255-1-AP | Download protocol |
IF protocol for NCAM1/CD56 antibody 14255-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Med Selective modulation of the androgen receptor AF2 domain rescues degeneration in spinal bulbar muscular atrophy. | ||
Cell Metab Multiplexed In Situ Imaging Mass Cytometry Analysis of the Human Endocrine Pancreas and Immune System in Type 1 Diabetes. | ||
Sci Adv Gene therapy with AR isoform 2 rescues spinal and bulbar muscular atrophy phenotype by modulating AR transcriptional activity. | ||
J Clin Invest Thioredoxin activity confers resistance against oxidative stress in tumor-infiltrating NK cells. | ||