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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NCAM1/CD56 Polyclonal antibody

NCAM1/CD56 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31536-1-AP

Synonyms

CD56, Ncam1, NCAM 1, Ncam, E NCAM

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCAM1/CD56 Polyclonal antibody (31536-1-AP)

    WB analysis using 31536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31536-1-AP (NCAM1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NCAM1/CD56 Polyclonal antibody (31536-1-AP)

    IHC staining of mouse brain using 31536-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31536-1-AP (NCAM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NCAM1/CD56 Polyclonal antibody (31536-1-AP)

    IHC staining of mouse brain using 31536-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31536-1-AP (NCAM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NCAM1/CD56 Polyclonal antibody (31536-1-AP)

    IHC staining of mouse cerebellum using 31536-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31536-1-AP (NCAM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NCAM1/CD56 Polyclonal antibody (31536-1-AP)

    IHC staining of mouse cerebellum using 31536-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31536-1-AP (NCAM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31536-1-AP targets NCAM1/CD56 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name neural cell adhesion molecule 1
Calculated molecular weight119 kDa
Observed molecular weight120 kDa, 140 kDa, 180 kDa
Gene symbol Ncam1
Gene ID (NCBI) 17967
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).

Protocols

Product Specific Protocols
WB protocol for NCAM1/CD56 antibody 31536-1-APDownload protocol
IHC protocol for NCAM1/CD56 antibody 31536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols