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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NCAM1/CD56 Monoclonal antibody, PBS Only

NCAM1/CD56 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1E8C9

Cat no : 60238-1-PBS

Synonyms

CD56, NCAM1, 1E8C9, NCAM 1, NCAM

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using NCAM1/CD56 Monoclonal antibody (60238-1-Ig)

    WB analysis of fetal human brain using 60238-1-Ig (same clone as 60238-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60238-1-Ig (NCAM1/CD56 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using NCAM1/CD56 Monoclonal antibody (60238-1-Ig)

    WB analysis of human brain using 60238-1-Ig (same clone as 60238-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using NCAM1/CD56 Monoclonal antibody (60238-1-Ig)

    WB analysis of C6 using 60238-1-Ig (same clone as 60238-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 60238-1-Ig (NCAM1/CD56 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using NCAM1/CD56 Monoclonal antibody (60238-1-Ig)

    WB analysis of pig brain using 60238-1-Ig (same clone as 60238-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60238-1-Ig (NCAM1/CD56 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NCAM1/CD56 Monoclonal antibody (60238-1-Ig)

    IHC staining of human lung cancer using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NCAM1/CD56 Monoclonal antibody (60238-1-Ig)

    IHC staining of human lung cancer using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using NCAM1/CD56 Monoclonal antibody (60238-1-Ig)

    IHC staining of human colon using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using NCAM1/CD56 Monoclonal antibody (60238-1-Ig)

    IHC staining of human colon using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon tissue using NCAM1/CD56 Monoclonal antibody (60238-1-Ig)

    IF Staining of human colon using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using NCAM1/CD56 antibody (60238-1-Ig, Clone: 1E8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using NCAM1/CD56 Monoclonal antibody (60238-1-Ig)

    IF Staining of SH-SY5Y using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using NCAM1/CD56 antibody (60238-1-Ig, Clone: 1E8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60238-1-PBS targets NCAM1/CD56 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NCAM1/CD56 fusion protein Ag5732 相同性解析による交差性が予測される生物種
Full Name neural cell adhesion molecule 1
Calculated molecular weight 95 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC047244
Gene symbol NCAM1
Gene ID (NCBI) 4684
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).