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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NCAM1/CD56 Recombinant antibody, PBS Only

NCAM1/CD56 Recombinant Antibody for IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5N5

Cat no : 82715-3-PBS

Synonyms

CD56, NCAM1, 5N5, NCAM 1, NCAM

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using NCAM1/CD56 Recombinant antibody (82715-3-RR)

    IHC staining of human tonsillitis using 82715-3-RR (same clone as 82715-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82715-3-RR (NCAM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using NCAM1/CD56 Recombinant antibody (82715-3-RR)

    IHC staining of human tonsillitis using 82715-3-RR (same clone as 82715-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82715-3-RR (NCAM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NCAM1/CD56 Recombinant antibody (82715-3-RR)

    IHC staining of human colon cancer using 82715-3-RR (same clone as 82715-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82715-3-RR (NCAM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NCAM1/CD56 Recombinant antibody (82715-3-RR)

    IHC staining of human colon cancer using 82715-3-RR (same clone as 82715-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82715-3-RR (NCAM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using NCAM1/CD56 Recombinant antibody (82715-3-RR)

    IF Staining of human tonsillitis using 82715-3-RR (same clone as 82715-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using NCAM1/CD56 antibody (82715-3-RR, Clone: 5N5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82715-3-RR

    Affinity and Kinetic Characterization of 82715-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82715-3-RR against Human NCAM1/CD56 were performed. The affinity constant is 1.45 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82715-3-PBS targets NCAM1/CD56 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NCAM1/CD56 fusion protein Ag5528 相同性解析による交差性が予測される生物種
Full Name neural cell adhesion molecule 1
Calculated molecular weight 95 kDa
GenBank accession numberBC047244
Gene symbol NCAM1
Gene ID (NCBI) 4684
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isoforms have also been described (PMID: 1856291).