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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCAM2 Recombinant antibody (83494-6-RR)

    WB analysis using 83494-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83494-6-RR (NCAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NCAM2 Recombinant antibody (83494-6-RR)

    IF Staining of mouse brain using 83494-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NCAM2 antibody (83494-6-RR, Clone: 240398D9 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83494-6-RR

    Affinity and Kinetic Characterization of 83494-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83494-6-RR against Human NCAM2 were performed. The affinity constant is 4.48 nM.

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NCAM2 Recombinant antibody

NCAM2 Recombinant Antibody for WB, IF-P, ELISA
Cat No. 83494-6-RR
Clone No.240398D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

NCAM21, NCAM-2, NCAM 2, N CAM 2, 240398D9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inLNCaP cells, mouse brain tissue
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83494-6-RR targets NCAM2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NCAM2 fusion protein Ag4821 相同性解析による交差性が予測される生物種
Full Name neural cell adhesion molecule 2
Calculated molecular weight 93 kDa
Observed molecular weight125 kDa
GenBank accession numberBC052946
Gene Symbol NCAM2
Gene ID (NCBI) 4685
RRIDAB_3671121
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15394
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mammalian NCAM family has two members, NCAM1 and NCAM2 (PMID: 9729879; 20439718). Neural cell adhesion molecule 2 (NCAM2, also known as NCAM21) is a single-pass type I membrane protein that is highly expressed in the brain, which may play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons or in neurodegenerative diseases such as Alzheimer's disease (PMID: 26611261). NCAM2 has two isoforms: NCAM2.1 bearing transmembrane and cytoplasmic domains and the shorter NCAM2.2 isoform, which is GPI-anchored (PMID: 9334170; 9221781). The molecular weight of NCAM2 is around 125 kDa and 93 kDa (PMID: 9334170; 3171685).

Protocols

Product Specific Protocols
WB protocol for NCAM2 antibody 83494-6-RRDownload protocol
IF protocol for NCAM2 antibody 83494-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols