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  • Featured Product
  • KD/KO Validated

NCAPD3 Polyclonal antibody, PBS Only

NCAPD3 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16828-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using NCAPD3 Polyclonal antibody (16828-1-AP)

    WB analysis of HepG2 using 16828-1-AP (same clone as 16828-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NCAPD3 Polyclonal antibody (16828-1-AP)

    WB analysis using 16828-1-AP (same clone as 16828-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NCAPD3 Polyclonal antibody (16828-1-AP)

    WB analysis using 16828-1-AP (same clone as 16828-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NCAPD3 Polyclonal antibody (16828-1-AP)

    WB analysis of HeLa using 16828-1-AP (same clone as 16828-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using NCAPD3 Polyclonal antibody (16828-1-AP)

    IP experiment of HeLa using 16828-1-AP (same clone as 16828-1-PBS)

    IP result of anti-NCAPD3 (IP:16828-1-AP, 4ug; Detection:16828-1-AP 1:500) with HeLa cells lysate 600ug. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NCAPD3 Polyclonal antibody (16828-1-AP)

    IF Staining of HepG2 using 16828-1-AP (same clone as 16828-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16828-1-AP (NCAPD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16828-1-PBS targets NCAPD3 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCAPD3 fusion protein Ag10429 相同性解析による交差性が予測される生物種
Full Name non-SMC condensin II complex, subunit D3
Calculated molecular weight 1498 aa, 169 kDa
Observed molecular weight 165 kDa
GenBank accession numberBC011408
Gene symbol NCAPD3
Gene ID (NCBI) 23310
RRIDAB_2282528
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4 , but they contain different sets of non-SMC subunits. NCAPD3 is 1 of 3 non-SMC subunits that define condensin II ([PMID: 14532007).