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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NCAPG Polyclonal antibody

NCAPG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29933-1-AP

Synonyms

CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, FLJ12450, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCAPG Polyclonal antibody (29933-1-AP)

    WB analysis using 29933-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29933-1-AP (NCAPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NCAPG Polyclonal antibody (29933-1-AP)

    IHC staining of human ovary tumor using 29933-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29933-1-AP (NCAPG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NCAPG Polyclonal antibody (29933-1-AP)

    IHC staining of human ovary tumor using 29933-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29933-1-AP (NCAPG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29933-1-AP targets NCAPG in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCAPG fusion protein Ag32040 相同性解析による交差性が予測される生物種
Full Name non-SMC condensin I complex, subunit G
Calculated molecular weight 1015 aa, 114 kDa
Observed molecular weight131 kDa
GenBank accession numberBC068467
Gene symbol NCAPG
Gene ID (NCBI) 64151
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NCAPG antibody 29933-1-APDownload protocol
IHC protocol for NCAPG antibody 29933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols