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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Neurochondrin Polyclonal antibody

Neurochondrin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13187-1-AP

Synonyms

KIAA0607, NCDN, Neurochondrin

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Neurochondrin Polyclonal antibody (13187-1-AP)

    WB analysis using 13187-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13187-1-AP (Neurochondrin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Neurochondrin Polyclonal antibody (13187-1-AP)

    WB analysis of mouse brain using 13187-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13187-1-AP (Neurochondrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using Neurochondrin Polyclonal antibody (13187-1-AP)

    WB analysis of rat brain using 13187-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 13187-1-AP (Neurochondrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Neurochondrin Polyclonal antibody (13187-1-AP)

    WB analysis of human brain using 13187-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13187-1-AP (Neurochondrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Neurochondrin Polyclonal antibody (13187-1-AP)

    IHC staining of mouse brain using 13187-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13187-1-AP (Neurochondrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Neurochondrin Polyclonal antibody (13187-1-AP)

    IHC staining of mouse brain using 13187-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13187-1-AP (Neurochondrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using Neurochondrin Polyclonal antibody (13187-1-AP)

    IHC staining of human brain using 13187-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13187-1-AP (Neurochondrin antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using Neurochondrin Polyclonal antibody (13187-1-AP)

    IHC staining of human brain using 13187-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13187-1-AP (Neurochondrin antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13187-1-AP targets Neurochondrin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Neurochondrin fusion protein Ag3859 相同性解析による交差性が予測される生物種
Full Name neurochondrin
Calculated molecular weight 729 aa, 79 kDa
Observed molecular weight 79 kDa
GenBank accession numberBC024592
Gene symbol Neurochondrin
Gene ID (NCBI) 23154
RRIDAB_2877919
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Neurochondrin antibody 13187-1-APDownload protocol
IHC protocol for Neurochondrin antibody 13187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

J Cell Sci

Neurochondrin interacts with the SMN protein suggesting a novel mechanism for spinal muscular atrophy pathology.

Authors - Luke W Thompson
View Article