• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NCF4 Polyclonal antibody

NCF4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14648-1-AP

Synonyms

P40PHOX, p40 phox, Neutrophil cytosol factor 4, NCF-4, NCF 4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of L02 cells using NCF4 Polyclonal antibody (14648-1-AP)

    WB analysis of L02 using 14648-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 14648-1-AP (NCF4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using NCF4 Polyclonal antibody (14648-1-AP)

    WB analysis of L02 using 14648-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 14648-1-AP (NCF4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using NCF4 Polyclonal antibody (14648-1-AP)

    IHC staining of human heart using 14648-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14648-1-AP (NCF4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using NCF4 Polyclonal antibody (14648-1-AP)

    IHC staining of human heart using 14648-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14648-1-AP (NCF4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NCF4 Polyclonal antibody (14648-1-AP)

    IF Staining of U2OS using 14648-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NCF4 antibody (14648-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inL02 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

14648-1-AP targets NCF4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCF4 fusion protein Ag6241 相同性解析による交差性が予測される生物種
Full Name neutrophil cytosolic factor 4, 40kDa
Calculated molecular weight 39 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC002798
Gene symbol NCF4
Gene ID (NCBI) 4689
RRIDAB_2251237
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NCF4, also named as p40phox, was the last NADPH oxidase subunit to be discovered, and although there is evidence of its both positive and negative effects on oxidase function. Catalog#14648-1-AP is a rabbit polyclonal antibody raised against the full-length of human NCF4.The MW of this protein is 40 kDa, and this antibody specially recognises the 40 kDa protein.

Protocols

Product Specific Protocols
WB protocol for NCF4 antibody 14648-1-APDownload protocol
IHC protocol for NCF4 antibody 14648-1-APDownload protocol
IF protocol for NCF4 antibody 14648-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
rat

BMC Pulm Med

Simvastatin attenuates silica-induced pulmonary inflammation and fibrosis in rats via the AMPK-NOX pathway

Authors - Cunxiang Bo
View Article
ratIF

CNS Neurosci Ther

Neutrophil migration participates in the side effect of recombinant human tissue plasminogen activator

Authors - Yuyou Huang
View Article