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KD/KO Validated

NCK1 Polyclonal antibody

Name: NCK1 Polyclonal antibody
Brand: Proteintech
SKU: 15247-1-AP

NCK1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15247-1-AP

Synonyms

SH2/SH3 adapter protein NCK-alpha, SH2/SH3 adapter protein NCK1, NCKalpha, Nck-1, NCK alpha

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 15247-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15247-1-AP (NCK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15247-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15247-1-AP (NCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15247-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15247-1-AP (NCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 15247-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 15247-1-AP (NCK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 15247-1-AP
IP result of anti-NCK1 (IP:15247-1-AP, 4ug; Detection:15247-1-AP 1:500) with mouse brain tissue lysate 2640ug.
IHC staining of human breast cancer using 15247-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15247-1-AP (NCK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15247-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15247-1-AP (NCK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15247-1-AP
Immunofluorescent analysis of Hela cells, using NCK1 antibody 15247-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
FC experiment of HeLa using 15247-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.4 ug NCK1 Polyclonal antibody (15247-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 15247-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, mouse liver tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 5 publications below
IHC	See 1 publications below
IP	See 1 publications below

Product Information

15247-1-AP targets NCK1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NCK1 fusion protein Ag0979 相同性解析による交差性が予測される生物種
Full Name	NCK adaptor protein 1
Calculated molecular weight	43 kDa
Observed molecular weight	43 kDa
GenBank accession number	BC006403
Gene symbol	NCK1
Gene ID (NCBI)	4690
RRID	AB_2150428
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NCK family adaptor proteins function to couple tyrosine phosphorylation signals to regulate actin cytoskeletal reorganization that leads to cell motility. Cytoplasmic protein NCK1 (or NCK alpha) is an adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. NCK1 binds a number of intracellular proteins and influences various signaling pathways including GTPase-activating protein of Ras (RasGAP), which is responsible for the down-regulation of Ras. NCK1 is recently reported to regulate the UPR, which secondary to obesity impairs glucose homeostasis and INS actions.

Protocols

Product Specific Protocols
WB protocol for NCK1 antibody 15247-1-AP	Download protocol
IHC protocol for NCK1 antibody 15247-1-AP	Download protocol
IF protocol for NCK1 antibody 15247-1-AP	Download protocol
IP protocol for NCK1 antibody 15247-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Signal Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis. Authors - Fan Zhang KD Validated View Article
human	WB,IP	Mol Immunol The adapter proteins ADAP and Nck cooperate in T cell adhesion. Authors - Marcus Lettau KD Validated View Article
human	WB,IHC	Mol Biol Rep STAT3-induced NCK1 elevation promotes migration of triple-negative breast cancer cells via regulating ERK1/2 signaling Authors - Peina He KD Validated View Article
human	WB	Cancer Biomark Long noncoding RNA LINC00885 upregulates NCK1 to promote cell viability and migration of triple-negative breast cancer cells through sponging miR-654-3p Authors - Peina He View Article
human	WB	Cell Microbiol The Legionella pneumophila effector WipA disrupts host F-actin polymerisation by hijacking phosphotyrosine signalling. Authors - Lei He View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NCK1 Polyclonal antibody

NCK1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 15247-1-AP

WB analysis of mouse brain using 15247-1-AP

WB analysis of mouse liver using 15247-1-AP

WB analysis of rat brain using 15247-1-AP

IP experiment of mouse brain using 15247-1-AP

IHC staining of human breast cancer using 15247-1-AP

IHC staining of human breast cancer using 15247-1-AP

IF Staining of HeLa using 15247-1-AP

FC experiment of HeLa using 15247-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis.

The adapter proteins ADAP and Nck cooperate in T cell adhesion.

STAT3-induced NCK1 elevation promotes migration of triple-negative breast cancer cells via regulating ERK1/2 signaling

Long noncoding RNA LINC00885 upregulates NCK1 to promote cell viability and migration of triple-negative breast cancer cells through sponging miR-654-3p

The Legionella pneumophila effector WipA disrupts host F-actin polymerisation by hijacking phosphotyrosine signalling.