NCKAP1 Polyclonal antibody
NCKAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 12140-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain |
| Positive IHC detected in | human gliomas tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IHC | See 3 publications below |
Product Information
12140-1-AP targets NCKAP1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NCKAP1 fusion protein Ag2788 相同性解析による交差性が予測される生物種 |
| Full Name | NCK-associated protein 1 |
| Calculated molecular weight | 129 kDa |
| Observed molecular weight | 115-125 kDa |
| GenBank accession number | BC015025 |
| Gene symbol | NCKAP1 |
| Gene ID (NCBI) | 10787 |
| RRID | AB_10666284 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NCK-associated protein 1 (NCKAP1), also known as NAP1 or HEM2, is encoded by an apoptosis-related gene human NAP1, the expression of which is strongly down-regulated in sporadic Alzheimer's disease (AD). Human NAP1 is predominantly expressed in neuronal cells. Antisense oligo DNA of human NAP1 transcripts was found to induce apoptosis of neuronal cells. Loss of NAP1 function disrupts neuronal differentiation. Likewise, NAP1 plays an essential role in facilitating neuronal cytoskeletal changes underlying the postmigratory differentiation of cortical neurons, a critical step in functional wiring of the cortex. Human NAP1 is also proved to be an orthologue of rat Nap1 which binds to the adaptor molecule NCK in signal transduction.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NCKAP1 antibody 12140-1-AP | Download protocol |
| IHC protocol for NCKAP1 antibody 12140-1-AP | Download protocol |
| IF protocol for NCKAP1 antibody 12140-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Nudel is crucial for the WAVE complex assembly in vivo by selectively promoting subcomplex stability and formation through direct interactions. | ||
Curr Biol Parallel kinase pathways stimulate actin polymerization at depolarized mitochondria. | ||
FASEB J Tropomyosin-related kinase C (TrkC) enhances podocyte migration by ERK-mediated WAVE2 activation. | ||
Biochem J Selenoprotein S is Involved in Maintenance and Transport of Multiprotein Complexes. | ||
World J Gastroenterol Abelson interactor 1 splice isoform-L plays an anti-oncogenic role in colorectal carcinoma through interactions with WAVE2 and full-length Abelson interactor 1. |
