Validation Data Gallery
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Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
10454-1-AP targets HEM1; NCKAP1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HEM1; NCKAP1L fusion protein Ag0706 相同性解析による交差性が予測される生物種 |
| Full Name | NCK-associated protein 1-like |
| Calculated molecular weight | 128 kDa |
| GenBank accession number | BC001604 |
| Gene Symbol | NCKAP1L |
| Gene ID (NCBI) | 3071 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55160 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Membrane-associated protein HEM1 encoded by human NCKAP1L (Nck-associated protein 1-like) gene belongs to the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. HEM1 is characterized as hematopoietic cell-specific actin regulatory protein. HEM1 is a component of the "WAVE" complex, which signals downstream of activated Rac to stimulate F-actin polymerization in response to immuno-receptor signaling. Therefore, HEM1 may be essential for most active processes dependent on reorganization of the actin cytoskeleton in immune cells.
Publications
| Species | Application | Title |
|---|---|---|
Gene Potential predictive value of immune-related genes FUCA1 and NCKAP1L for osteosarcoma metastasis |