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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCKAP5 Polyclonal antibody (31199-1-AP)

    WB analysis using 31199-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31199-1-AP (NCKAP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NCKAP5 Polyclonal antibody (31199-1-AP)

    WB analysis using 31199-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31199-1-AP (NCKAP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NCKAP5 Polyclonal antibody (31199-1-AP)

    IF Staining of U2OS using 31199-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NCKAP5 antibody (31199-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

NCKAP5 Polyclonal antibody

NCKAP5 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31199-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

NAP5, Peripheral clock protein, Nck associated protein 5, NAP 5, ERIH2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31199-1-AP targets NCKAP5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCKAP5 fusion protein Ag35092 相同性解析による交差性が予測される生物種
Full Name Nck-associated protein 5
Calculated molecular weight209 kDa
Observed molecular weight250-260 kDa
GenBank accession numberNM_207363
Gene Symbol NAP5
Gene ID (NCBI) 344148
RRIDAB_3669897
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO14513
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NCKAP5 (Nck-associated protein 5), also known as NAP5. NCKAP5 is predicted to be located in cells, especially in nucleoli, Golgi apparatus, cell membrane and cytoplasm. It is widely expressed in many tissues, especially in lung and kidney, and in more than 20 other tissues. And the expression of NCKAP5 was enhanced in oligodendrocytes, oligodendrocyte precursor cells and astrocytes. It may interact with other protein, such as NPM1 and centromere protein complex, and regulate accurate chromosome segregation and distribution. NCKAP5 may be involved in the formation and development of tumors, especially in the process of tumor variation such as ovary and breast cancer. In addition, NCKAP5 may be a diagnostic biomarker in non-small cell lung cancer (NSCLC), which is related to the infiltration of activated B cells, regulatory T cells (Tregs), neutrophils and dendritic cells (PMID: 36628881). The molecular weight of NCKAP5 is 208 kDa, it also has some protein modifications.

Protocols

Product Specific Protocols
WB protocol for NCKAP5 antibody 31199-1-APDownload protocol
IF protocol for NCKAP5 antibody 31199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols