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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NCOA1/SRC-1 Polyclonal antibody

NCOA1/SRC-1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51114-1-AP

Synonyms

bHLHe42, bHLHe74, F SRC 1, KAT13A, NCoA 1, NCOA1, nuclear receptor coactivator 1, Protein Hin 2, RIP160, SRC 1, SRC1, Steroid receptor coactivator 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using NCOA1/SRC-1 Polyclonal antibody (51114-1-AP)

    WB analysis of mouse testis using 51114-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 51114-1-AP (NCOA1/SRC-1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using NCOA1/SRC-1 Polyclonal antibody (51114-1-AP)

    WB analysis of PC-3 using 51114-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 51114-1-AP (NCOA1/SRC-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using NCOA1/SRC-1 Polyclonal antibody (51114-1-AP)

    WB analysis of MCF-7 using 51114-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 51114-1-AP (NCOA1/SRC-1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using NCOA1/SRC-1 Polyclonal antibody (51114-1-AP)

    IHC staining of human testis using 51114-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 51114-1-AP (NCOA1/SRC-1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using NCOA1/SRC-1 Polyclonal antibody (51114-1-AP)

    IHC staining of human testis using 51114-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 51114-1-AP (NCOA1/SRC-1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inmouse testis tissue, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51114-1-AP targets NCOA1/SRC-1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name nuclear receptor coactivator 1
Calculated molecular weight 1441 aa, 157 kDa
Observed molecular weight 140-160 kDa
GenBank accession numberBC111533
Gene symbol SRC 1/NCOA1
Gene ID (NCBI) 8648
RRIDAB_2150905
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NCOA1/SRC-1 antibody 51114-1-APDownload protocol
IHC protocol for NCOA1/SRC-1 antibody 51114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols