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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCOA2 Polyclonal antibody (30962-1-AP)

    WB analysis using 30962-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30962-1-AP (NCOA2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NCOA2 Polyclonal antibody (30962-1-AP)

    IF Staining of U2OS using 30962-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NCOA2 antibody (30962-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NCOA2 Polyclonal antibody (30962-1-AP)

    IF Staining of HepG2 using 30962-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NCOA2 antibody (30962-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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NCOA2 Polyclonal antibody

NCOA2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30962-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NCoA 2, KAT13C, hTIF2, GRIP1, Class E basic helix-loop-helix protein 75

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HepG2 cells, Jurkat cells, LNCaP cells
Positive IF/ICC detected inHepG2 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

30962-1-AP targets NCOA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCOA2 fusion protein Ag33768 相同性解析による交差性が予測される生物種
Full Name nuclear receptor coactivator 2
Calculated molecular weight 1464 aa, 159 kDa
Observed molecular weight160-180 kDa
GenBank accession numberBC114383
Gene Symbol NCOA2
Gene ID (NCBI) 10499
RRIDAB_3669796
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ15596
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NCOA2 antibody 30962-1-APDownload protocol
IF protocol for NCOA2 antibody 30962-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Phytomedicine

Yiqi Huayu Jiedu formula inhibits JAK2/STAT3-mediated partial EMT in treating chronic atrophic gastritis

Authors - Weifeng Yu
View Article