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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NCOA3 Polyclonal antibody

NCOA3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29587-1-AP

Synonyms

AIB1, SRC3, CAGH 16, bHLHe42, AIB-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCOA3 Polyclonal antibody (29587-1-AP)

    WB analysis using 29587-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29587-1-AP (NCOA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NCOA3 Polyclonal antibody (29587-1-AP)

    WB analysis using 29587-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29587-1-AP (NCOA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NCOA3 Polyclonal antibody (29587-1-AP)

    IHC staining of human lung cancer using 29587-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29587-1-AP (NCOA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using NCOA3 Polyclonal antibody (29587-1-AP)

    IHC staining of human skin cancer using 29587-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29587-1-AP (NCOA3 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NCOA3 Polyclonal antibody (29587-1-AP)

    IHC staining of human lung cancer using 29587-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29587-1-AP (NCOA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inDaudi cells, HEK-293 cells, Hela cells, Jurkat cells, K-562 cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman lung cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29587-1-AP targets NCOA3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCOA3 fusion protein Ag30633 相同性解析による交差性が予測される生物種
Full Name nuclear receptor coactivator 3
Calculated molecular weight 1424 aa, 155 kDa
Observed molecular weight150-160 kDa
GenBank accession numberBC119001
Gene symbol NCOA3
Gene ID (NCBI) 8202
RRIDAB_2918328
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NCOA3, also named as AIB1, SRC3 and TRAM1, belongs to the SRC/p160 nuclear receptor coactivator family. NCOA3 is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. NOCA3 plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). NCOA3 also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.

Protocols

Product Specific Protocols
WB protocol for NCOA3 antibody 29587-1-APDownload protocol
IHC protocol for NCOA3 antibody 29587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols