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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NCOA3 Polyclonal antibody, PBS Only

NCOA3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29587-1-PBS

Synonyms

AIB1, SRC3, CAGH 16, bHLHe42, AIB-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCOA3 Polyclonal antibody (29587-1-AP)

    WB analysis using 29587-1-AP (same clone as 29587-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29587-1-AP (NCOA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NCOA3 Polyclonal antibody (29587-1-AP)

    WB analysis using 29587-1-AP (same clone as 29587-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29587-1-AP (NCOA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NCOA3 Polyclonal antibody (29587-1-AP)

    IHC staining of human lung cancer using 29587-1-AP (same clone as 29587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29587-1-AP (NCOA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using NCOA3 Polyclonal antibody (29587-1-AP)

    IHC staining of human skin cancer using 29587-1-AP (same clone as 29587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29587-1-AP (NCOA3 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NCOA3 Polyclonal antibody (29587-1-AP)

    IHC staining of human lung cancer using 29587-1-AP (same clone as 29587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29587-1-AP (NCOA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29587-1-PBS targets NCOA3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCOA3 fusion protein Ag30633 相同性解析による交差性が予測される生物種
Full Name nuclear receptor coactivator 3
Calculated molecular weight 1424 aa, 155 kDa
Observed molecular weight150-160 kDa
GenBank accession numberBC119001
Gene symbol NCOA3
Gene ID (NCBI) 8202
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NCOA3, also named as AIB1, SRC3 and TRAM1, belongs to the SRC/p160 nuclear receptor coactivator family. NCOA3 is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. NOCA3 plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). NCOA3 also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.