Validation Data Gallery
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Tested Applications
| Positive IP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
25241-1-AP targets NCOA6 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NCOA6 fusion protein Ag18737 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear receptor coactivator 6 |
| Calculated molecular weight | 2063 aa, 219 kDa |
| Observed molecular weight | 68 kDa, 219 kDa, 110 kDa |
| GenBank accession number | BC136272 |
| Gene Symbol | NCOA6 |
| Gene ID (NCBI) | 23054 |
| RRID | AB_2879983 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14686 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NCOA6 (Nuclear receptor coactivator 6), also named as AIB3 or ANTP, is a 2063 amino acid protein, which contains two LXXLL motifs. NCOA6 localizes in the nucleus and is highly expressed in brain, prostate, testis and ovary. NCOA6 directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. The molecular weight of NCOA6 is 219 kDa, but we always detected 68 kDa protein by western blot.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NCOA6 antibody 25241-1-AP | Download protocol |
| IP protocol for NCOA6 antibody 25241-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol TRACERx analysis identifies a role for FAT1 in regulating chromosomal instability and whole-genome doubling via Hippo signalling | ||
Mol Ther Nucleic Acids Downregulated lncRNA HOXA11-AS Affects Trophoblast Cell Proliferation and Migration by Regulating RND3 and HOXA7 Expression in PE. | ||
Mol Pharmacol Alterations of Histone Modifications Contribute to Pregnane X Receptor-mediated Induction of CYP3A4 by Rifampicin. | ||
Cancer Med Nuclear receptor coactivator 6 (NCoA6) promotes cell proliferation, migration, and invasion in pancreatic cancer
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