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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NCOR2 Polyclonal antibody

NCOR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29952-1-AP

Synonyms

CTG repeat protein 26, CTG26, N CoR2, NCOR2, Nuclear receptor corepressor 2, SMAP270, SMRT, SMRTE, SMRTE tau, T3 receptor associating factor, TNRC14, TRAC, TRAC 1, TRAC1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCOR2 Polyclonal antibody (29952-1-AP)

    WB analysis using 29952-1-AP

    MOLT-4 cells were subjected to Tris-Acetate gel system followed by western blot with 29952-1-AP (NCOR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NCOR2 Polyclonal antibody (29952-1-AP)

    WB analysis using 29952-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29952-1-AP (NCOR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NCOR2 Polyclonal antibody (29952-1-AP)

    IHC staining of human prostate cancer using 29952-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29952-1-AP (NCOR2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NCOR2 Polyclonal antibody (29952-1-AP)

    IHC staining of human prostate cancer using 29952-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29952-1-AP (NCOR2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using NCOR2 Polyclonal antibody (29952-1-AP)

    IF Staining of A549 using 29952-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using NCOR2 antibody (29952-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inJurkat cells, MOLT-4 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29952-1-AP targets NCOR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCOR2 fusion protein Ag30727 相同性解析による交差性が予測される生物種
Full Name nuclear receptor co-repressor 2
Calculated molecular weight 274 kDa
Observed molecular weight274 kDa
GenBank accession numberNM_006312
Gene symbol NCOR2
Gene ID (NCBI) 9612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NCOR2, nuclear receptor corepressor 2 , is a transcriptional corepressor that that contains several nuclear receptor-interacting domains. NCOR2/SMRT acts as a platform protein, facilitating the recruitment of histone deacetylases to the DNA promoters bound by its interacting transcription factors (PMID: 9150137). NCOR2 mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription (PMID: 23081895).

Protocols

Product Specific Protocols
WB protocol for NCOR2 antibody 29952-1-APDownload protocol
IHC protocol for NCOR2 antibody 29952-1-APDownload protocol
IF protocol for NCOR2 antibody 29952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Minor Spliceosomal 65K/RNPC3 Interacts with ANKRD11 and Mediates HDAC3-Regulated Histone Deacetylation and Transcription

Authors - Chen-Hui Li
View Article