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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCS1 Monoclonal antibody (67616-1-Ig)

    WB analysis using 67616-1-Ig (same clone as 67616-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67616-1-Ig (NCS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NCS1 Monoclonal antibody (67616-1-Ig)

    IHC staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67616-1-Ig (NCS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NCS1 Monoclonal antibody (67616-1-Ig)

    IHC staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67616-1-Ig (NCS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NCS1 Monoclonal antibody (67616-1-Ig)

    IF Staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCS1 antibody (67616-1-Ig, Clone: 2F10C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51282-1

    Cytometric bead array standard curve of MP51282-1

    Cytometric bead array standard curve of MP51282-1, FREQ Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67616-1-PBS. Detection antibody: 67616-2-PBS. Standard:Ag29808. Range: 1.563-100 ng/mL.

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NCS1 Monoclonal antibody, PBS Only (Capture)

NCS1 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 67616-1-PBS
Clone No.2F10C8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

FREQ, FLUP, Frequenin-like protein, Frequenin like protein, frequenin homolog (Drosophila)

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67616-1-PBS targets NCS1 as part of a matched antibody pair:

MP51282-1: 67616-1-PBS capture and 67616-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NCS1 fusion protein Ag29808 相同性解析による交差性が予測される生物種
Full Name frequenin homolog (Drosophila)
Calculated molecular weight 22 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC004856
Gene Symbol NCS1
Gene ID (NCBI) 23413
RRIDAB_2882819
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62166
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.